E-GEOD-25077 - Gene expression in response to progestin R5020

Status
Released on 14 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (7)
Description
Breast cancer cell lines containing the progesterone receptor respond to progestins, altering expression of a subset of genes. In a previously published experiment of inducible knock-down of histone H1 isoforms with an shRNA-expression lentivector (GSE12299), we modified the breast cancer cell line T47D with different vectors. Here, we explored response to progestin R5020 of control cells generated with the empty shRNA-expression vector. Stable breast cancer-derived cell lines containing the empty vector for inducible shRNA expression were grown for two days in the absence of serum. Progestin R5020 or vehicle was added for 6 hours, in duplicate, and RNA was extracted for microarray hybridization.
Experiment type
transcription profiling by array 
Contacts
Lauro Sumoy <lsumoy@imppc.org>, Albert Jordan, Mónica Sancho
Citation
Progestins activate 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) in breast cancer cells. Novellasdemunt L, Obach M, Mill�n-Ari�o L, Manzano A, Ventura F, Rosa JL, Jordan A, Navarro-Sabate A, Bartrons R. , Europe PMC 22115192
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-25077.idf.txt
Sample and data relationshipE-GEOD-25077.sdrf.txt
Processed data (1)E-GEOD-25077.processed.1.zip
Array designA-MEXP-931.adf.txt
Links