E-GEOD-2342 - Dexamethasone responses in BRC-Abl (p210) transformed murine B-cells

Status
Released on 25 February 2006, last updated on 2 June 2014
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (2)
Description
For generation of pre-B cell lines, bone marrow from tibias and femurs of 4 to 8 week old C56Bl/6 mice (wt and vav-Gfi1b transgene) was infected with retroviral stocks of different BCR-Abl constructs (MSCV-GFP-IRESp210 or pSRa-p210-tkneo) and plated at a density of 5E+06 per 6 cm dish. All pre-B cells were fed twice a week and analyzed after they became stroma independent. Cells received 1E-08 M dexamethasone or vehicle for 12 hours prior to RNA extraction using TRIzol. mRNAs were converted into doublestranded cDNA using a T7-promoter tagged oligodT primer and transcribed by T7 RNA polymerase in the presence of biotinylated NTPs. Targets were hybridized to Affymetrix MOE430A arrays, washed, stained and scanned (GeneChip scanner 2500) as recommended by the manufacturer. Images were processed either by MAS5.0 (global scaling, TGT=1000) or RMAExpress (quantile normalization and background substraction) software tools. By comparing the gene expression profiles of dexamethasone and non-induced cells, dexamethasone target genes were identified Keywords: ordered
Experiment type
transcription profiling by array 
Contacts
Katharina Fiolka, Lothar Vassen, Ludger Klein-Hitpass, Stefan Mahlmann, Tarik Möröy
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-2342.idf.txt
Sample and data relationshipE-GEOD-2342.sdrf.txt
Raw data (1)E-GEOD-2342.raw.1.zip
Processed data (1)E-GEOD-2342.processed.1.zip
Array designA-AFFY-23.adf.txt
Links