E-GEOD-2283 - Primary human neuroblastoma

Status
Released on 12 May 2005, last updated on 27 March 2012
Organism
Homo sapiens
Samples (372)
Arrays (2)
Protocols (1)
Description
Reference1: Ohira M, Oba S, Nakamura Y, Isogai E, Kaneko S, Nakagawa A, Hirata T, Kubo H, Goto T, Yamada S, Yoshida Y, Fuchioka M, Ishii S, Nakagawara A. Expression profiling of human primary Expression profiling using a tumor-specific cDNA microarray predicts the prognosis of intermediate risk neuroblastomas. Cancer Cell. 2005 Apr;7(4):337-50. To predict the prognosis of neuroblastoma patients and choose a better therapeutic protocol, we developed a cDNA microarray carrying 5340 genes obtained from primary neuroblastomas and examined 136 tumor samples. We made a probabilistic output statistical classifier that provided a high accuracy in prognosis prediction (89% at 5 years) and a highly reliable method to validate it. Kaplan-Meier analysis indicated that the patients in an intermediate group defined by existing markers are divided by microarray into two further groups with 5 year survivals for 36% and 89% of patients (p < 10(-4)), i.e., with unfavorably and favorably predicted neuroblastomas, respectively. According to these results, we developed a gene subset chip for a clinical tool, for which our classifier exhibited 88% prediction accuracy. Datasets: neuroblastomas by CCC-NB200-Chip: GSM42338-GSM42387 Expression profiling of human primary neuroblastomas by CCC-NB5000-Chip: GSM42391-GSM42526 Keywords = Neuroblastoma
Experiment type
unknown experiment type 
Contacts
Akira Nakagawara <akiranak@chiba-cc.jp>, Atsuko Nakagawa, Eriko Isogai, Hiroyuki Kubo, Miki Ohira, Misa Fuchioka, Saichi Yamada, Setsuko Kaneko, Shigeyuki Oba, Shin Ishii, Takahiro Hirata, Takeshi Goto, Yasuko Yoshida, Yohko Nakamura
Citation
Expression profiling using a tumor-specific cDNA microarray predicts the prognosis of intermediate risk neuroblastomas. Ohira M, Oba S, Nakamura Y, Isogai E, Kaneko S, Nakagawa A, Hirata T, Kubo H, Goto T, Yamada S, Yoshida Y, Fuchioka M, Ishii S, Nakagawara A.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2283.idf.txt
Sample and data relationshipE-GEOD-2283.sdrf.txt
Processed data (1)E-GEOD-2283.processed.1.zip
Array designsA-GEOD-1874.adf.txt, A-GEOD-1875.adf.txt
Links