E-GEOD-2241 - Transcription profiling time series of HOXA5 induction in human Hs578T cell line
Submitted on 3 February 2005, released on 25 August 2007, last updated on 27 March 2012
HOXA5 inducible cell line Hs578T was established and cultured as described previously (MCB 2004. 24:924-935). 2×106 cells were seeded onto a 10-cm cell culture dish at 24h before induction. Two batches of RNA from HOXA5-inducible cells (0 h, 6 h and 9 h) were purified for repeating the microarray hybridization experiments once using Affymatrix Chips.Total RNA was extracted using TRIzol reagent (Gibco BRL, Life Technologies, Grand Island, NY) and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
transcription profiling by array, co-expression, individual genetic characteristics, time series
Identification of transcriptional targets of HOXA5. Hexin Chen, Ethel Rubin, Huiping Zhang, Seung Chung, Charles C Jie, Elizabeth Garrett, Shyam Biswal, Saraswati Sukumar. J Biol Chem 280(19):19373-80 (2005)