E-GEOD-22094 - Transcription profiling by array of bone marrow cells from wild type, Fancc-deficient, Fancg-deficient, and doubly deficient (Fancc/Fancg) mice

Status
Released on 30 July 2010, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (6)
Description
Seeking to challenge the current dogma that the nuclear core-complex proteins function in an entirely epistatic manner, Dr. Clapp's group developed a new double-knockout mouse nullizygous for Fancc and Fancg. Because the hematopoietic phenotype was more severe than single knockout mice, we reasoned that transcriptomal differences would exist and lead to the identification of molecular defects unique to each FA gene. RNA was purified from unfractionated and uncultured bone marrow cells from three types of Fanconi anemia gene knockout mice: nullizygous for Fancc, Fancg, or both Fancc and Fancg.. Three wild type C57Bl/6 mice served as controls. Each of three marrow samples provided one RNA sample (the RNA samples were not pooled).
Experiment type
transcription profiling by array 
Contacts
Citation
Genetic disruption of both Fancc and Fancg in mice recapitulates the hematopoietic manifestations of Fanconi anemia. Pulliam-Leath AC, Ciccone SL, Nalepa G, Li X, Si Y, Miravalle L, Smith D, Yuan J, Li J, Anur P, Orazi A, Vance GH, Yang FC, Hanenberg H, Bagby GC, Clapp DW.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-22094.idf.txt
Sample and data relationshipE-GEOD-22094.sdrf.txt
Raw data (1)E-GEOD-22094.raw.1.zip
Processed data (1)E-GEOD-22094.processed.1.zip
Array designA-AFFY-45.adf.txt
Links