E-GEOD-19879 - Thymine starvation and limitation in Escherichia coli thyA- cells
Submitted on 13 January 2010, released on 14 May 2010, last updated on 2 May 2014
Transcriptional profiling of responses of E. coli thymine auxotrophs to i) growth under sub-optimal thymine concentration (TLM) and ii) lethal conditions under complete thymine starvation (TLD). Thymine limitation: Time series following shift to limiting thymine concentrations (15, 30, 45, 60 and 90 mins) - single replicate. Thymine starvation: Time series following shift to zero thymine medium (15, 30, 45, 60 and 90 mins) - 2 biological replicates at each time point.
transcription profiling by array
Dipen Sangurdekar <email@example.com>, Arkady B Khodursky, Bree L Hamann, Dipen P Sangurdekar
Thymineless death is associated with loss of essential genetic information from the replication origin. Sangurdekar DP, Hamann BL, Smirnov D, Srienc F, Hanawalt PC, Khodursky AB.