E-GEOD-19879 - Thymine starvation and limitation in Escherichia coli thyA- cells

Status
Submitted on 13 January 2010, released on 14 May 2010, last updated on 2 May 2014
Organism
Escherichia coli
Samples (30)
Array (1)
Protocols (9)
Description
Transcriptional profiling of responses of E. coli thymine auxotrophs to i) growth under sub-optimal thymine concentration (TLM) and ii) lethal conditions under complete thymine starvation (TLD). Thymine limitation: Time series following shift to limiting thymine concentrations (15, 30, 45, 60 and 90 mins) - single replicate. Thymine starvation: Time series following shift to zero thymine medium (15, 30, 45, 60 and 90 mins) - 2 biological replicates at each time point.
Experiment type
transcription profiling by array 
Contacts
Dipen Sangurdekar <dps@genomics.princeton.edu>, Arkady B Khodursky, Bree L Hamann, Dipen P Sangurdekar
Citation
Thymineless death is associated with loss of essential genetic information from the replication origin. Sangurdekar DP, Hamann BL, Smirnov D, Srienc F, Hanawalt PC, Khodursky AB.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19879.idf.txt
Sample and data relationshipE-GEOD-19879.sdrf.txt
Raw data (1)E-GEOD-19879.raw.1.zip
Processed data (1)E-GEOD-19879.processed.1.zip
Array designA-GEOD-3503.adf.txt
R ExpressionSetE-GEOD-19879.eSet.r
Links