E-GEOD-19784 - Transcription profiling by array of human bone marrow plasma cells from multiple myeloma patients
Released on 27 June 2010, last updated on 23 March 2012
In order to identify relevant, molecularly defined subgroups in Multiple Myeloma (MM), gene expression profiling (GEP) was performed on purified CD138+ plasma cells of 320 newly diagnosed myeloma patients included in the Dutch-Belgian/German HOVON-65/ GMMG-HD4 trial using Affymetrix Gene Chip U133 plus 2.0 arrays. Hierarchical clustering identified 10 distinct subgroups. Bone marrow plasma cell samples were obtained from 320 newly diagnosed multiple myeloma patients included in a large multicenter, prospective, randomized phase III trial (HOVON65/GMMG-HD4). Purified myeloma plasma cells samples with a monoclonal plasma cell purity > 80% were used for analysis.
transcription profiling by array
Annemiek Broyl, Dirk Hose, Hartmut Goldschmidt, Mark van Duin, Pieter Sonneveld, Yvonne de Knegt
Gene expression profiling for molecular classification of multiple myeloma in newly diagnosed patients. Broyl A, Hose D, Lokhorst H, de Knegt Y, Peeters J, Jauch A, Bertsch U, Buijs A, Stevens-Kroef M, Beverloo HB, Vellenga E, Zweegman S, Kersten MJ, van der Holt B, El Jarari L, Mulligan G, Goldschmidt H, van Duin M, Sonneveld P. , Europe PMC 20574050