E-GEOD-19732 - Transcription profiling by array of mouse sublines of Swiss 3T3 fibroblasts with varying potential for adipogenesis

Status
Released on 21 February 2010, last updated on 30 April 2015
Organism
Mus musculus
Samples (20)
Array (1)
Protocols (8)
Description
In order to identify the molecular mechanisms controlling preadipocyte commitment we derived new sublines of Swiss 3T3 fibroblasts with varying potential for adipogenesis. Swiss 2, Swiss 8, Swiss 9, and Swiss 19 differentiate into fat cells while Swiss 3, Swiss 5, and Swiss 22 have a lower propensity to differentiate into fat cells. The overall goal was to identify genes whose expression in the fibroblast state correlates with the ultimate potential of the cells to undergo adipogenesis 4 preadipose fibroblast cell lines (Swiss 2, Swiss 8, Swiss 9, and Swiss 19 in triplicate or duplicate) were compared to 3 non-adipogenic fibroblast cell lines (Swiss 3, Swiss 5, Swiss 22 in triplicate)
Experiment type
transcription profiling by array 
Contacts
Citation
Transcriptional control of preadipocyte determination by Zfp423. Gupta RK, Arany Z, Seale P, Mepani RJ, Ye L, Conroe HM, Roby YA, Kulaga H, Reed RR, Spiegelman BM.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19732.idf.txt
Sample and data relationshipE-GEOD-19732.sdrf.txt
Raw data (1)E-GEOD-19732.raw.1.zip
Processed data (1)E-GEOD-19732.processed.1.zip
Array designA-AFFY-45.adf.txt
Links