E-GEOD-18530 - High-Resolution Nucleosome Mapping Reveals Transcription-Dependent Promoter Packaging

Status
Released on 15 October 2009, last updated on 21 August 2013
Organism
Saccharomyces cerevisiae
Samples (5)
Protocols (4)
Description
Genome-wide mapping of nucleosomes has revealed a great deal about the relationships between chromatin structure and control of gene expression, and has led to mechanistic hypotheses regarding the rules by which chromatin structure is established. High-throughput sequencing has recently become the technology of choice for chromatin mapping studies, yet analysis of these experiments is still in its infancy. Here, we introduce a pipeline for analyzing deep sequencing maps of chromatin structure and apply it to data from S. cerevisiae. We analyze digestion series where nucleosomes are isolated from under- and over-digested chromatin. We find that certain classes of nucleosomes are unusually susceptible or resistant to overdigestion, with promoter nucleosomes easily digested and mid-coding region nucleosomes being quite stable. We find evidence for highly sensitive nucleosomes located within “nucleosome-free regions,” suggesting that these regions are not always completely naked but instead are likely associated with easily-digested nucleosomes. Finally, since RNA polymerase is the dominant energy-consuming machine that operates on the chromatin template, we analyze changes in chromatin structure when RNA polymerase is inactivated via a temperature-sensitive mutation. We find evidence that RNA polymerase plays a role in nucleosome eviction at promoters, and is also responsible for retrograde shifts in nucleosomes during transcription. Loss of RNA polymerase results in a relaxation of chromatin structure to more closely match in vitro nucleosome positioning preferences. Together, these results provide analytical tools and experimental guidance for nucleosome mapping experiments, and help disentangle the interlinked processes of transcription and chromatin packaging. Keywords: Genomic Solexa/Illumina sequencing of mononucleosomes. Titration series: gel-purified mononucleosomal DNA from three different titration levels – underdigested, typical digestion, and overdigested. RP021 series: MNase-seq at 0, 20, and 120 minutes after shifting these cells from 25 C to 37 C.
Experiment type
ChIP-seq 
Contacts
Assaf Weiner <assafwe@cs.huji.ac.il>, Amanda Hughes, Moran Yassour, Nir Friedman, Oliver J Rando
Citation
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-18530.idf.txt
Sample and data relationshipE-GEOD-18530.sdrf.txt
Processed data (1)E-GEOD-18530.processed.1.zip
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