E-GEOD-18181 - Meiotic time-course expression profile

Status
Submitted on 20 September 2009, released on 22 May 2010, last updated on 1 May 2014
Organism
Saccharomyces cerevisiae SK1
Samples (54)
Array (1)
Protocols (15)
Description
To investigate the dynamics of the meiotic transcriptome and to ensure meiotic synchrony of our samples, we measured RNA abundance in vegetative cells, respiratory (pre-meiotic) cells and in synchronously sporulating cells at various times after transfer to sporulation media (SM). SK1 yeast strain SHy002 (MATa/MATα ho::LYS2/ho::LYS2 leu2::hisG/leu2::hisG lys2/lys2 ura3/ura3)
Meiotic Time Course with samples collected during vegetative growth (YPD), respiratory growth (pre-meiotic/YPA 8 hours) and 0 hr, 1.5hr, 3hr, 4.5hr, 6hr, 9hr and 12hr after transfer to sporulation media (SM).
3 separate biological replicates were used for each condition/time-point.
Total RNA from each sample was hybridized competitively with a genomic DNA common reference.
The expression analysis was carried out from the same biological samples as the FAIRE analysis.
Experiment type
transcription profiling by array 
Contacts
Jason D Lieb <jlieb@bio.unc.edu>, Sean Erik Hanlon <shanlon@email.unc.edu>, Gregory P Copenhaver, Luke E Berchowitz, Sean E Hanlon
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18181.idf.txt
Sample and data relationshipE-GEOD-18181.sdrf.txt
Raw data (1)E-GEOD-18181.raw.1.zip
Processed data (1)E-GEOD-18181.processed.1.zip
Array designA-GEOD-4414.adf.txt
R ExpressionSetE-GEOD-18181.eSet.r
Links