E-GEOD-17639 - The human reticulocyte transcriptome (HG-U133_Plus2.0)

Status
Released on 30 November 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
Description
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133Plus 2.0 arrays were hybridized with probe from total RNA isolated from blood sampled from 6 umbilical cords and 6 healthy adult humans. Peripheral blood from 6 normal adults and 6 human umbilical cords were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133Plus 2.0 arrays.
Experiment type
transcription profiling by array 
Contacts
Jeffery Lynn Miller <jm7f@nih.gov>, Jeffery L Miller, Sung-Ho Goh
Citation
Let-7 microRNAs are developmentally regulated in circulating human erythroid cells. Noh SJ, Miller SH, Lee YT, Goh SH, Marincola FM, Stroncek DF, Reed C, Wang E, Miller JL. , Europe PMC 19939273
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-17639.idf.txt
Sample and data relationshipE-GEOD-17639.sdrf.txt
Raw data (1)E-GEOD-17639.raw.1.zip
Processed data (1)E-GEOD-17639.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-17639.eSet.r
Links