E-GEOD-17375 - Gene expression in colon cancer stem cells (CSC) cultures identified by Wnt signaling levels

Status
Released on 1 December 2009, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (6)
Description
Primary colon CSC cultures were transduced with a Wnt responsive construct (TOP-GFP) and were single cell cloned. 10% highest and lowest TOP-GFP cell fractions were FACS sorted and arrayed. Primary colon CSC cultures with a responsive construct (TOP-GFP): high and low TOP-GFP cell fractions. 4 samples.
Experiment type
transcription profiling by array 
Contacts
Louis Vermeulen <l.vermeulen@amc.uva.nl>, Felipe de Sousa E Melo, Jan_Paul Medema, Louis Vermeulen
Citation
Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP. , Europe PMC 20418870
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-17375.idf.txt
Sample and data relationshipE-GEOD-17375.sdrf.txt
Raw data (1)E-GEOD-17375.raw.1.zip
Processed data (1)E-GEOD-17375.processed.1.zip
Array designA-AFFY-44.adf.txt
Links