E-GEOD-1727 - Transcription profiling of mouse fetal liver feeder layer and endothelial progenitor cells

Status
Submitted on 3 September 2004, released on 15 December 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (7)
Array (1)
Protocols (2)
Description
While others have reported that fetal liver contains a population of endothelial progenitors based on expression of cell surface markers or culture assays, this is the first proof of a CD31+Sca1+ progenitor by demonstrating highly efficient in vivo angiogenesis and a direct connection to the host vasculature. We have developed a novel isolation method based on collagenase digestion and culture on a fetal liver-derived feeder layer and demonstrate that the feeder cells or their supernatants are required for endothelial progenitor survival and proliferation. Proteogenomic profiling of the endothelial progenitors and the feeder cells was done with tandem mass spectrometry proteomics using MudPIT and gene transcript expression profiling using high density DNA microarrays. This approach identified a number of gene transcripts, proteins and candidate growth factor pathways that are likely to be involved in endothelial progenitor growth, differentiation and angiogenesis.
Experiment types
transcription profiling by array, cell type comparison, co-expression
Contact
Citation
Isolation and angiogenesis by endothelial progenitors in the fetal liver. Stephanie Cherqui,Sunil M Kurian,Olivier Schussler,Johannes A Hewel,John R Yates,Daniel R Salomon. Stem cells (Dayton, Ohio) :44-54 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-1727.idf.txt
Sample and data relationshipE-GEOD-1727.sdrf.txt
Raw data (1)E-GEOD-1727.raw.1.zip
Processed data (1)E-GEOD-1727.processed.1.zip
Array designA-AFFY-36.adf.txt
Links