E-GEOD-1724 - Transcription profiling of human lung fibroblasts isolated from pulmonary fibrosis patients and treated with TGF beta

Status
Submitted on 2 September 2004, released on 4 August 2007, last updated on 10 June 2011
Organism
Homo sapiens
Samples (18)
Array (1)
Description
The response to a 4 hour treatment with TGFbeta (4 ng/ml) was evaluated in lung fibroblasts derived from three controls (normal periphery of resected tumor), open lung biopsies from three patients with idiopathic pulmonary fibrosis (usual interstitial pneumonia pattern on biopsy) and from three patients with fibrosing alveolitis associated with systemic sclerosis (fibrotic non specific interstitial pneumonia pattern on biopsy). Lung fibroblasts were grown to confluence in DMEM with 10% fetal calf serum. At confluence, lung fibroblasts were serum-deprived overnight, and exposed to either 4 ng/ml of activated TGF-ß1 (R&D Systems) or serum-free culture medium with 0.1% BSA for four hours.
Experiment types
transcription profiling by array, co-expression, compound treatment, disease state
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-1724.idf.txt
Sample and data relationshipE-GEOD-1724.sdrf.txt
Processed data (1)E-GEOD-1724.processed.1.zip
Array designA-AFFY-1.adf.txt
Links