E-GEOD-16647 - Gene expression profiling of NCAM-deficient mouse brain

Status
Released on 1 December 2009, last updated on 2 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Analysis of brains of mice lacking the neural cell adhesion molecule NCAM (Ncam-/-) in comparison to wild-type mice of same age and genetic background (Ncam+/+). NCAM-deficient mice exhibit deficits in long-term potentiation and spatial learning, as well as increased intermale aggression. We performed a microarray analysis of adult NCAM-deficient mouse brains to identify molecular targets which might underly the phenotype of NCAM-deficient mice. We analyzed the gene expression profiles in 2-3 months old (young adult) mouse brains, with both the Ncam-/- and the Ncam+/+ group consisting of three individuals each. Wild-type and NCAM-deficient animals had the same genetic background. RNA was prepared from total brain.
Experiment type
transcription profiling by array 
Contacts
Thomas Streichert, Yong W Wong
Citation
Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression. Meier ID, Bernreuther C, Tilling T, Neidhardt J, Wong YW, Schulze C, Streichert T, Schachner M. , Europe PMC 20110269
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16647.idf.txt
Sample and data relationshipE-GEOD-16647.sdrf.txt
Raw data (1)E-GEOD-16647.raw.1.zip
Processed data (1)E-GEOD-16647.processed.1.zip
Array designA-AFFY-6.adf.txt
Links