E-GEOD-16098 - Transcription profiling of human total or cytoplamsic RNA from peripheral blood mononuclear cells expressing HTLV-I p30 to asses the ability of p30 to regulate the export of cellular transcripts to the cytoplasm
Submitted on 13 May 2009, released on 16 October 2009, last updated on 10 June 2011
The Human T-cell Leukemia Virus (HTLV)-type-I non-structural protein p30 plays an important role in virus transmission and gene regulation. p30 has been documented to inhibit the export of certain viral mRNA transcripts from the nucleus to the cytoplasm. This nuclear retainment of RNA molecules essentially results in gene silencing, where protein products are not produced. Considering this unique function of p30, we used microarray analysis to assess the ability of p30 to inhibit not only the regulation of transcription of cellular genes, but also the ability of p30 to regulate the export of cellular transcripts to the cytoplasm. Experiment Overall Design: Total or cytoplamsic RNA from peripheral blood mononuclear cells expressing HTLV-I p30 was isolated and analyzed by microarray analysis, in comparison with mock-transcuced cells.
transcription profiling by array, unknown experiment type