E-GEOD-1595 - Transcription profiling of human bladder smooth muscle cells subjected to cyclic stretch-relaxation
Submitted on 25 July 2004, released on 4 August 2007, last updated on 27 March 2012
The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched).
transcription profiling by array, co-expression, stimulus or stress
Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells. Rosalyn M Adam, Samuel H Eaton, Carlos Estrada, Ashish Nimgaonkar, Shu-Ching Shih, Lois E H Smith, Isaac S Kohane, Darius Bagli, Michael R Freeman. Physiol Genomics 20(1):36-44 (2004)