E-GEOD-1595 - Transcription profiling of human bladder smooth muscle cells subjected to cyclic stretch-relaxation

Status
Submitted on 25 July 2004, released on 4 August 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (1)
Description
The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched).
Experiment types
transcription profiling by array, co-expression, stimulus or stress
Contact
Citation
Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells. Rosalyn M Adam, Samuel H Eaton, Carlos Estrada, Ashish Nimgaonkar, Shu-Ching Shih, Lois E H Smith, Isaac S Kohane, Darius Bagli, Michael R Freeman. Physiol Genomics 20(1):36-44 (2004)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-1595.idf.txt
Sample and data relationshipE-GEOD-1595.sdrf.txt
Processed data (1)E-GEOD-1595.processed.1.zip
Array designA-AFFY-33.adf.txt
Links