E-GEOD-15949 - Transcription profiling of human primary macrophages experiencing hypoxia reveals hypoxia inducible factors 1 and 2 are important transcriptional effectors
Submitted on 4 May 2009, released on 11 May 2009, last updated on 3 May 2014
Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used microarrays to detail the hypoxia upregulated gene in human primary macrophages. Experiment Overall Design: Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators.
transcription profiling by array, unknown experiment type