E-GEOD-15949 - Transcription profiling of human primary macrophages experiencing hypoxia reveals hypoxia inducible factors 1 and 2 are important transcriptional effectors

Status
Submitted on 4 May 2009, released on 11 May 2009, last updated on 3 May 2014
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (6)
Description
Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used microarrays to detail the hypoxia upregulated gene in human primary macrophages. Experiment Overall Design: Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-15949.idf.txt
Sample and data relationshipE-GEOD-15949.sdrf.txt
Raw data (1)E-GEOD-15949.raw.1.zip
Processed data (1)E-GEOD-15949.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-15949.eSet.r
Links