E-GEOD-15624 - Transcription profiling of mouse T cells treated with halofuginone to investigate regulation of Y cell gene expression by Halofuginone (HF)

Status
Submitted on 10 April 2009, released on 19 April 2009, last updated on 10 June 2011
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (5)
Description
T cell differentiation to the Th17 effector subset requires stimulation through the T cell and co-stimulatory receptors, together with cytokine stimulation by TGFb and IL-6. The small molecule halofuginone (HF) inhibits Th17 cell development and induces a pattern of stress-regulated gene expression that mimics amino acid starvation. We used global transcript profiling to ask how halofuginone modulates gene expression induced during T cell activaiton and Th17 differentiation Experiment Overall Design: Purified mouse CD4+ CD25- T cells were activated under Th17 polarizing cytokine conditions and treated with either halofuginone or its inactive derivative, MAZ1310, for 3- or 6-hours.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-15624.idf.txt
Sample and data relationshipE-GEOD-15624.sdrf.txt
Raw data (1)E-GEOD-15624.raw.1.zip
Processed data (1)E-GEOD-15624.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-15624.eSet.r
Links