E-GEOD-1518 - Transcription profiling of human umbilical vein endothelial cells (HUVEC) exposed to sheer stress and intraluminal pressure

Status
Submitted on 30 June 2004, released on 4 August 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (2)
Description
Intact living conduit vessels (umbilical veins) were exposed to normal or high intraluminal pressure, or low or high shear stress in combination with a physiological level of the other force. We used a unique vascular ex vivo perfusion system. After six hours of perfusion endothelial cells were isolated from the stimulated vessels and RNA was extracted. RNA from 16 experiments from each stimulation were pooled and analyzed in duplicate DNA microarrays.
Experiment types
transcription profiling by array, co-expression, stimulus or stress
Contact
Citation
Differential global gene expression response patterns of human endothelium exposed to shear stress and intraluminal pressure. Maria Andersson, Lena Karlsson, Per-Arne Svensson, Erik Ulfhammer, Mikael Ekman, Margareta Jernas, Lena M S Carlsson, Sverker Jern. J Vasc Res 42(5):441-52 (2005)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-1518.idf.txt
Sample and data relationshipE-GEOD-1518.sdrf.txt
Raw data (1)E-GEOD-1518.raw.1.zip
Processed data (1)E-GEOD-1518.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-1518.eSet.r
Links