E-GEOD-14380 - Transcription profiling of human colorectal cancer cell lines transfected with homeobox gene CDX1 cDNA to identfiy downstream targets

Status
Submitted on 12 January 2009, released on 23 January 2009, last updated on 1 May 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (4)
Description
This study is to identify downstream targets of homeobox gene CDX1. The study assayed the expression of 2 pairs of stably transfected colorectal cancer cell lines: The CDX1 nonexpressing CRC cell line HCT116 was stably transfected with either CDX1 cDNA in the pRC/CMV expression vector (HCT116-CDX1) or with vector control (HCT116-Vec). The CDX1-expressing CRC cell line LS174T was similarly transfected with either a pSilencer vector containing a short sequence of CDX1 siRNA (LS174T-siRNA) , or a pSilencer vector containing a scrambled siRNA sequence as a control (LS174T-Vec). Experiment Overall Design: 2 pairs of colorectal cancer cell lines were used for comparison
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-14380.idf.txt
Sample and data relationshipE-GEOD-14380.sdrf.txt
Raw data (1)E-GEOD-14380.raw.1.zip
Processed data (1)E-GEOD-14380.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-14380.eSet.r
Links