E-GEOD-14056 - Co-immunoprecipitation of RNAs with RNA helicase A (RHA)

Status
Submitted on 19 December 2008, released on 19 December 2008, last updated on 10 June 2011
Organism
Chlorocebus aethiops
Samples (3)
Array (1)
Protocols (8)
Description
Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from duplicate experiments with samples obtained in two independent RNA immunoprecipitations identified 407 transcripts that co-immunoprecipitate with FLAG RHA. Keywords: gene expression array-based Since N-terminal FLAG tagged RHA specifically co-immunoprecipitates PCE-containing mRNAs COS cells were transfected with a CMV-FLAG-RHA construct. Cytoplasmic lysates were pooled and immunoprecipitated with anti-FLAG beads. RNA was extracted from the immunoprecipitate and used to probe rhesus macaque Affimetrix expression arrays. Steady state mRNA levels were monitored on a separate array probed with total RNA.
Experiment type
other 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-14056.idf.txt
Sample and data relationshipE-GEOD-14056.sdrf.txt
Raw data (1)E-GEOD-14056.raw.1.zip
Processed data (1)E-GEOD-14056.processed.1.zip
Array designA-AFFY-145.adf.txt
R ExpressionSetE-GEOD-14056.eSet.r
Links