E-GEOD-1347 - Transcription profiling by array of human bone marrow samples after storage at room temperature for up to 48 hours after aspiration

Status
Released on 28 July 2007, last updated on 3 April 2012
Organism
Homo sapiens
Samples (9)
Array (1)
Description
Impact of pre-analytical handling on Bone Marrow (BM) mRNA Gene Expression: We have investigated the impact of RNA extraction protocols and time delays (24 and 48h) between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48h after aspiration. However, storage of BM has dramatic effects on mRNA expression of individual transcripts.
Experiment types
transcription profiling by array, co-expression, optimization, time series
Contact
Citation
Impact of pre-analytical handling on bone marrow mRNA gene expression. Stephen Breit, Matthias Nees, Ulrike Schaefer, Margit Pfoersich, Christian Hagemeier, Martina Muckenthaler, Andreas E Kulozik. , Europe PMC 15238145
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-1347.idf.txt
Sample and data relationshipE-GEOD-1347.sdrf.txt
Processed data (1)E-GEOD-1347.processed.1.zip
Array designA-AFFY-1.adf.txt
Links