E-GEOD-13419 - High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and ddm1 mutant
Released on 2 February 2009, last updated on 27 June 2012
DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, implying that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis, as in cancer, or meiosis, leading to epiallelic variation. Here, we demonstrate the existence of an efficient and faithful mechanism that protects against transgenerational loss of DNA methylation in the plant Arabidopsis. This process is specific to the subset of heavily methylated genomic repeats that are targeted by the RNAi machinery, and does not spread into flanking regions. Remethylation is often progressive over two to four sexual generations. This differential and incremental correction of epigenetic defects may preserve genome stability while increasing adaptive opportunities. 2 samples examined: wild type, and ddm1 mutant.
RNA-seq of non coding RNA
Alexis Sarazin, C Cruaud, Constance Ciaudo, Fabiana Heredia, Felipe K Teixeira, François Roudier, J Poulain, Manel Esteller, Maria Berdasco, Mario F Fraga, Martine Boccara, Olivier Voinnet, P Wincker, Vincent Colot
A role for RNAi in the selective correction of DNA methylation defects. Teixeira FK, Heredia F, Sarazin A, Roudier F, Boccara M, Ciaudo C, Cruaud C, Poulain J, Berdasco M, Fraga MF, Voinnet O, Wincker P, Esteller M, Colot V. , Europe PMC 19179494