E-GEOD-12710 - Transcription profiling by array of human peripheral blood samples collected into lithium heparin tubes followed by transfer to PAXgene or Tempus tubes

Status
Released on 13 November 2008, last updated on 16 May 2012
Organism
Homo sapiens
Samples (20)
Array (1)
Protocols (4)
Description
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of RNA isolation methods on gene expression profiles. We demonstrated that peripheral blood RNA isoaltion can critically impact differential expression results, particularly in the clinical setting where fold-change differences are typically small and there is inherent variability within biological corhorts Experiment Overall Design: Comparison of 5 healthy control samples drawn directly in PAXgene or Tempus tubes vs. the same 5 healthy control samples drawn in Li Heparin tubes with no PHA stimulation.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation. Asare AL, Kolchinsky SA, Gao Z, Wang R, Raddassi K, Bourcier K, Seyfert-Margolis V. , Europe PMC 18847473
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12710.idf.txt
Sample and data relationshipE-GEOD-12710.sdrf.txt
Raw data (2)E-GEOD-12710.raw.1.zip, E-GEOD-12710.raw.2.zip
Processed data (1)E-GEOD-12710.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-12710.eSet.r
Links