E-GEOD-12709 - Transcription profiling of human peripheral blood samples incubated for 3 hours at room temperature with or without addition of PHA

Status
Released on 13 November 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (28)
Array (1)
Protocols (4)
Description
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of RNA isolation methods on gene expression profiles. We demonstrated that peripheral blood RNA isoaltion can critically impact differential expression results, particularly in the clinical setting where fold-change differences are typically small and there is inherent variability within biological corhorts Experiment Overall Design: Whole blood from 7 healthy individuals was collected using Li Heparin tubes, and was incubated for 3 hours at room temperature with or without addition of 25 ug/mL of PHA. After incubation, samples were subsequently transferred to either Tempus or PAXgene tubes.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Zhong Gao
Citation
Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation. Adam L Asare, Svetlana A Kolchinsky, Zhong Gao, Richard Wang, Khadir Raddassi, Katarzyna Bourcier, Vicki Seyfert-Margolis.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-12709.idf.txt
Sample and data relationshipE-GEOD-12709.sdrf.txt
Raw data (2)E-GEOD-12709.raw.1.zip, E-GEOD-12709.raw.2.zip
Processed data (1)E-GEOD-12709.processed.1.zip
Array designA-AFFY-44.adf.txt
Links