E-GEOD-12444 - siRNA profiling of human primary prostate stromal cells knocked down for FOXF2

Status
Released on 13 November 2008, last updated on 24 March 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (6)
Description
To identify the genes and pathways regulated by FOXF2, we investigated potential FOXF2 gene targets by microarray analyses of primary prostate stromal cells (PrSC) in which FOXF2 was knocked down by siRNA. 190 differentially expressed genes were selected, of which 104 genes were more highly expressed in PrSC cells treated with FOXF2 siRNA and 86 were more highly expressed in PRSC cells treated with negative control siRNA. Experiment Overall Design: In each experiment, we compared gene expression of PrSC cells treated with FOXF2 siRNA versus PrSC cells treated with negative control siRNA, in a total of 6 affymetrix arrays. 190 differentially expressed genes were selected (ratio negative control siRNA/siRNA ≥ 2log |0.8| as average in all arrays).
Experiment types
transcription profiling by array, RNAi profiling by array
Contact
Citation
The FOXF2 pathway in the human prostate stroma. van der Heul-Nieuwenhuijsen L, Dits N, Van Ijcken W, de Lange D, Jenster G.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-12444.idf.txt
Sample and data relationshipE-GEOD-12444.sdrf.txt
Raw data (1)E-GEOD-12444.raw.1.zip
Processed data (1)E-GEOD-12444.processed.1.zip
Array designA-AFFY-44.adf.txt
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