E-GEOD-12426 - DIDS protects against neuronal injury

Status
Submitted on 11 August 2008, released on 15 May 2010, last updated on 10 June 2011
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (10)
Description
Using long-oligo beadschip arrays, we examined the global gene expression profile in cultured hippocampal slices under the simulated ischemic condition with and without the presence of DIDS . Control-ACSF: cultured hippocampal slices were incubated with ACSF for 2 hours and were analysed on whole-genome expression chips to determine the gene expression profile. This group was used as controls for IS-treated groups and ACSF+DIDS-treated grous. IS: cultured hippocampal slices were treated wtih IS for 2 hours and the gene expression was analyzed and compared with the control group. IS+DIDS: cultured slices were treated with both IS and DIDS for 2 hours and the gene expression profile was determined. Differentially expression genes were identified by comparing with IS group. ACSF+DIDS: cultured slices were incubated with both ACSF and DIDS for 2 hours and gene expression profile was determined. This group was used as a control for IS+DIDS group. ACSF: artificial cerebral spinal fluid DIDS: 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid IS: ischemic solution
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12426.idf.txt
Sample and data relationshipE-GEOD-12426.sdrf.txt
Raw data (1)E-GEOD-12426.raw.1.zip
Processed data (1)E-GEOD-12426.processed.1.zip
Array designA-GEOD-6568.adf.txt
Links