E-GEOD-12379 - Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene

Status
Released on 8 August 2008, last updated on 2 June 2014
Organism
Mycobacterium tuberculosis
Samples (16)
Array (1)
Protocols (14)
Description
Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of four M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, the espR transposon mutant complemented with the wild type copy of the espR gene, and the espR transposon mutant complemented with an N-terminal FLAG espR gene.
Experiment type
transcription profiling by array 
Contacts
Jeffery Cox, Paolo Manzanillo, Sridharan Raghavan
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-12379.idf.txt
Sample and data relationshipE-GEOD-12379.sdrf.txt
Raw data (1)E-GEOD-12379.raw.1.zip
Processed data (1)E-GEOD-12379.processed.1.zip
Array designA-GEOD-6719.adf.txt
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