E-GEOD-12379 - Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene
Released on 8 August 2008, last updated on 2 June 2014
Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of four M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, the espR transposon mutant complemented with the wild type copy of the espR gene, and the espR transposon mutant complemented with an N-terminal FLAG espR gene.
transcription profiling by array
Jeffery Cox, Paolo Manzanillo, Sridharan Raghavan
Secreted transcription factor controls Mycobacterium tuberculosis virulence. Raghavan S, Manzanillo P, Chan K, Dovey C, Cox JS. , Europe PMC 18685700