E-GEOD-12356 - Transcription profiling of mouse bone marrow with Aiolos and OBF-1 deletions from 7 week old mice

Status
Submitted on 6 August 2008, released on 13 November 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (6)
Description
The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expression is deregulated in the pre-B cell compartment of these mice. In particular, we found that components of the pre-BCR, such as the surrogate light chain genes l5l5 and VpreB, fail to be efficiently silenced in double-mutant mice. Strikingly, developmentally regulated nuclear repositioning of the l5l5 gene is impaired in pre-B cells lacking OBF-1 and Aiolos. These studies uncover a novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function. OBF-1 or Aiolos or both were deleted and the gene expression profiles for these animals investigated using Affymetrix arrays Experiment Overall Design: Two control wildtype animals, then duplicates for each of the single Aiolos or OBF-1 mutants and further duplicates for the Aiolos/OBF-1 double mutants
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Silencing and nuclear repositioning of the lambda5 gene locus at the pre-b cell stage requires Aiolos and OBF-1. Alexander Karnowski, Chun Cao, Gabriele Matthias, Sebastian Carotta, Lynn M Corcoran, Inga-Lill Martensson, Jane A Skok, Patrick Matthias.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12356.idf.txt
Sample and data relationshipE-GEOD-12356.sdrf.txt
Raw data (1)E-GEOD-12356.raw.1.zip
Processed data (1)E-GEOD-12356.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-12356.eSet.r
Links