E-GEOD-12325 - Global gene expression analysis of Arabidopsis ppi2-1 mutant

Status
Submitted on 3 August 2008, released on 8 August 2008, last updated on 27 March 2012
Organism
Arabidopsis thaliana
Samples (2)
Protocols (4)
Description
This study aims at investigating the gene expression profile of Arabidopsis ppi2-1 mutant. Using Super-SAGE, we compared the gene expression profile of ppi2-1 mutant with that of wild-type Arabidopsis. To obtain the SuperSAGE libraries, we followed the original SuperSAGE protocol described by Matsumura and colleagues (Matsumura et al., 2003) with some modification. Total RNA was isolated from the 3rd to 6th leaves of Ws and the ppi2-1 mutant using RNAiso reagent. The SuperSAGE library was directly sequenced with a large-scale pyrosequencing method.
Experiment type
transcription profiling by SAGE 
Contacts
Hideo Matsumura, Ryohei Terauchi, Takehito Inaba, Tomohiro Kakizaki
Citation
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-12325.idf.txt
Sample and data relationshipE-GEOD-12325.sdrf.txt
Processed data (1)E-GEOD-12325.processed.1.zip
Links