E-GEOD-12310 - Transcription profiling of mouse normal lymphoid progenitors and MLL-AF4 leukemic lymphoblasts

Status
Submitted on 31 July 2008, released on 13 November 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (39)
Array (1)
Protocols (3)
Description
We created a mouse model where conditional expression of physiologic levels of an Mll-AF4 fusion oncogene induces development of acute lymphoblastic (ALL) or acute myeloid leukemias (AML). Immunophenotypic and gene expression analysis of the ALL cells demonstrated bone marrow replacement with B-precursor cells which express a gene expression profile that has significant overlap with profiles in human MLL-rearranged ALL. Experiment Overall Design: Total RNA from normal pro-B, pre-B, immature B and mature B lymphocytes, and from leukemic pre-B cells was isolated and hybridized to Affymetrix expresison microarrays.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
H3K79 methylation profiles define murine and human MLL-AF4 leukemias. Andrei V Krivtsov, Zhaohui Feng, Madeleine E Lemieux, Joerg Faber, Sridhar Vempati, Amit U Sinha, Xiaobo Xia, Jonathan Jesneck, Adrian P Bracken, Lewis B Silverman, Jeffery L Kutok, Andrew L Kung, Scott A Armstrong.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-12310.idf.txt
Sample and data relationshipE-GEOD-12310.sdrf.txt
Raw data (1)E-GEOD-12310.raw.1.zip
Processed data (1)E-GEOD-12310.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-12310.eSet.r
Links