E-GEOD-12229 - microRNA expression profiling among human embryonic stem cells, differerentiated EBs and adult cells

Status
Submitted on 23 July 2008, released on 17 April 2009, last updated on 3 May 2014
Organism
Homo sapiens
Samples (42)
Array (1)
Protocols (61)
Description
Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. We aimed to identify the unique signature of miRNAs in human embryonic stem cells. Keywords: cell type comparison design We performed microRNA expression profiling on 3 passages from 2 different hES cell lines (WA09 (H9) and BG01v) and 4 passages from 1 hES cell line (TE06 (I6)), EBs samples derived from WA09 cells at 4 different time points with replicates, and 5 types of adult cells (HUVEC, HMVEC, UASMC NHA, and LFB).
Experiment type
transcription profiling by array 
Contact
Citation
MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells. Ren J, Jin P, Wang E, Marincola FM, Stroncek DF.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12229.idf.txt
Sample and data relationshipE-GEOD-12229.sdrf.txt
Raw data (1)E-GEOD-12229.raw.1.zip
Processed data (1)E-GEOD-12229.processed.1.zip
Array designA-GEOD-7089.adf.txt
R ExpressionSetE-GEOD-12229.eSet.r
Links