E-GEOD-11983 - Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae

Status
Submitted on 2 July 2008, released on 4 July 2008, last updated on 1 May 2014
Organism
Saccharomyces cerevisiae
Samples (8)
Array (1)
Protocols (9)
Description
The Zap1 transcription factor is a central player in the response of yeast to changes in zinc status. Zap1 acts as both a positive and negative regulator of its target genes. We previously used transcriptome profiling with DNA microarrays to identify 46 potential Zap1 target genes in the yeast genome. In this study, we have used complementary methods to identify additional Zap1 target genes. With alternative growth conditions for the microarray experiments and a more sensitive motif identification algorithm, we have identified 31 new potential targets of Zap1 activation. Keywords: Yeast Saccharomyces cerevisiae whole genome array We compared cells overexpressing a constitutive allele of Zap1v.s. vector alone (E3) and wild type strain in severe zinc deficiency v.s. zinc-replete condition (E4)
Experiment type
transcription profiling by array 
Contacts
CHANG-YI WU <wu4@wisc.edu>, Amanda J Bird, Chang-Yi Wu, David Eide, Dennis R Winge, Lisa M Chung, Michael A Newton
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-11983.idf.txt
Sample and data relationshipE-GEOD-11983.sdrf.txt
Raw data (1)E-GEOD-11983.raw.1.zip
Processed data (1)E-GEOD-11983.processed.1.zip
Experiment designE-GEOD-11983.biosamples.png, E-GEOD-11983.biosamples.svg
Array designA-GEOD-7018.adf.txt
Links