E-GEOD-11961 - Gene expressions of memory B cells and other type of B cells
Released on 21 September 2012, last updated on 1 October 2012
To obtain insight into the genetic basis of the increase of functional activity of memory B cells over time, we compared the gene expression profiles of day 7 and day 40 NP-specific/IgG1 memory B cells, GC B cells and plasma cells in immunized WT mice and naïve B cells, before and after activation in vitro. Total RNAs in three replicates of NP-specific/IgG1 memory B cells, GC B cells and plasma cells in immunized WT mice at day 7 and 40 postimmunization and naïve follicular and marginal zone B cells, before and after activation in vitro, were amplified and labeled according to the Affymetrix's instructions. cRNA was hybridized to Mouse Genome 430 2.0 GeneChips (Affymetrix).
transcription profiling by array
Kaji Tomohiro <email@example.com>, Akiko Sugimoto, Atushi Hijikata, Mariko Okada, Osamu Ohara, Takeshi Nagashima, Toshitada Takemori