E-GEOD-11961 - Gene expressions of memory B cells and other type of B cells

Status
Released on 21 September 2012, last updated on 1 October 2012
Organism
Mus musculus
Samples (24)
Array (1)
Protocols (6)
Description
To obtain insight into the genetic basis of the increase of functional activity of memory B cells over time, we compared the gene expression profiles of day 7 and day 40 NP-specific/IgG1 memory B cells, GC B cells and plasma cells in immunized WT mice and naïve B cells, before and after activation in vitro. Total RNAs in three replicates of NP-specific/IgG1 memory B cells, GC B cells and plasma cells in immunized WT mice at day 7 and 40 postimmunization and naïve follicular and marginal zone B cells, before and after activation in vitro, were amplified and labeled according to the Affymetrix's instructions. cRNA was hybridized to Mouse Genome 430 2.0 GeneChips (Affymetrix).
Experiment type
transcription profiling by array 
Contacts
Kaji Tomohiro <t-kaji@rcai.riken.jp>, Akiko Sugimoto, Atushi Hijikata, Mariko Okada, Osamu Ohara, Takeshi Nagashima, Toshitada Takemori
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-11961.idf.txt
Sample and data relationshipE-GEOD-11961.sdrf.txt
Raw data (1)E-GEOD-11961.raw.1.zip
Processed data (1)E-GEOD-11961.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-11961.eSet.r
Links