E-GEOD-11932 - Transcription profiling of E. coli K-12 MG1655 and E. coli K-12 LVM100 delta 5 TA mutant effect on biofilm cell development

Status
Submitted on 29 June 2008, released on 9 February 2009, last updated on 1 May 2014
Organism
Escherichia coli
Samples (2)
Array (1)
Protocols (4)
Description
The role of six toxin-antitoxin (TA) systems on biofilm development was investigated (MazEF, RelBEF, ChpB, YefM-YoeB, DinJ-YafQ, and TomB-Hha). Although these TA systems were reported previously to not impact bacterial fitness, we found that biofilm formation is decreased by toxins and increased by anti-toxins, in part, through YjgK. Hence, one role of TA systems is to regulate biofilm formation. Experiment Overall Design: Strains: E. coli K-12 MG1655 and E. coli K-12 LVM100 delta 5 TA mutant Experiment Overall Design: Medium: LB Experiment Overall Design: Cells: biofilm cells on glass wool Experiment Overall Design: Time: 15 h Experiment Overall Design: Temperature: 37oC
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-11932.idf.txt
Sample and data relationshipE-GEOD-11932.sdrf.txt
Raw data (1)E-GEOD-11932.raw.1.zip
Processed data (1)E-GEOD-11932.processed.1.zip
Experiment designE-GEOD-11932.biosamples.png, E-GEOD-11932.biosamples.svg
Array designA-AFFY-56.adf.txt
R ExpressionSetE-GEOD-11932.eSet.r
Links