E-GEOD-11928 - Differential expression of inflammatory and immune response genes in rams experimentally infected with Brucella ovis

Status
Submitted on 27 June 2008, released on 2 July 2008, last updated on 1 May 2014
Organism
Ovis aries
Samples (12)
Array (1)
Protocols (12)
Description
Infection of sheep with Brucella ovis results in ovine brucellosis, a disease characterized by infertility in rams, abortion in ewes and increased perinatal mortality in lambs. During the course of the infection both the ovine immune response and host cell gene expression are modified. The objective of this research was to conduct a preliminary characterization of differential gene expression in rams experimentally infected with B. ovis by microarray hybridization and real-time RT-PCR. Six hybridizations were conducted using total RNA from three individual infected sheep at 15 and 60 days post infection. In each comparison, the control channels contained total RNA from each of the same three sheep at 0 days post infection. Ratios were calculated as B. ovis-infected sheep at 15 and 60 dpc versus uninfected animals at 0 dpc.
Experiment type
transcription profiling by array 
Contacts
Ruth Cecilia Galindo, Christian Gortazar, Clara M Marin, Jose de la Fuente, José M Blasco, Katherine M Kocan, María J de Miguel, Pilar M Muñoz, Ruth C Galindo
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11928.idf.txt
Sample and data relationshipE-GEOD-11928.sdrf.txt
Processed data (1)E-GEOD-11928.processed.1.zip
Experiment designE-GEOD-11928.biosamples.png, E-GEOD-11928.biosamples.svg
Array designA-GEOD-6954.adf.txt
Links