E-GEOD-11891 - Transcription profiling of mouse aorta-gonad-mesonephros(AGM) derived stromal cells

Status
Released on 26 October 2008, last updated on 12 October 2011
Organism
Mus musculus
Samples (9)
Arrays (3)
Protocols (2)
Description
A mouse AGM-derived cell line, AGM-s3, was shown to support the development of hematopoietic stem cells. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-s3, some of which were hematopoiesis supportive (s3-A9) and others which were non-supportive (s3-A7), and we analyzed the gene expression profiles by gene chip analysis. Experiment Overall Design: Genome-wide gene expression was examined using Affymetrix GeneChip array. Assays were performed according to the manufacturer's protocol. Total RNA was isolated from each stromal cell lines. We analysed 3 cell lines, AGM-s3-A9, AGM-s3-A7 and OP9. AGM-s3-A9 and OP9 are hematopoiesis supportive cell lines. AGM-s3-A7 is a hematopoiesis non-supportive cell line.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
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