E-GEOD-11806 - miRNA Microarray Profiling of Nine Different Normal Human Tissues
Submitted on 15 June 2008, released on 19 August 2008, last updated on 10 June 2011
We measured the expression of 470 human miRNAs in nine human tissues using Agilent DNA microarrays. miRNA microarrays were manufactured by Agilent Technologies (Santa Clara, CA), and contain 20-40 features targeting each of 470 human miRNAs (Agilent design ID 016436). Sequences of the 470 miRNAs were obtained from the Sanger miRBase, release 9.1. Labeling and hybridization of total RNA samples were performed according to the manufacturer’s protocol. 100ng total RNA was used as input into the labeling reaction, and the entire reaction was hybridized to the array for 20 hours at 55°C. The labeling and hybridizations of the nine human tissues were done 4-5 times using the same starting RNA sample for each tissue.
transcription profiling by array
Robert Ach, Bo Curry, Hui Wang, Robert A Ach
Measuring microRNAs: comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods. Ach RA, Wang H, Curry B.