E-GEOD-11696 - Gene expression profile of M.tuberculosis espR (Rv3849) mutant

Status
Submitted on 4 June 2008, released on 4 July 2008, last updated on 1 May 2014
Organism
Mycobacterium tuberculosis
Samples (18)
Array (1)
Protocols (9)
Description
Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of three M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, and the espR transposon mutant complemented with the espR gene
Experiment type
transcription profiling by array 
Contacts
Paolo Manzanillo <paolom@berkeley.edu>, Jeffery Cox, Sridharan Raghavan
Citation
Secreted transcription factor controls Mycobacterium tuberculosis virulence. Raghavan S, Manzanillo P, Chan K, Dovey C, Cox JS.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11696.idf.txt
Sample and data relationshipE-GEOD-11696.sdrf.txt
Raw data (1)E-GEOD-11696.raw.1.zip
Processed data (1)E-GEOD-11696.processed.1.zip
Experiment designE-GEOD-11696.biosamples.png, E-GEOD-11696.biosamples.svg
Array designA-GEOD-6719.adf.txt
Links