E-GEOD-1151 - CEA_SGF:E00004#TetOFF_Stat3F

Status
Submitted on 26 March 2004, released on 1 July 2004, last updated on 27 March 2012
Organism
Mus musculus
Samples (46)
Arrays (2)
Protocols (1)
Description
The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. Upon tetracycline removal but inthe presence of LIF (Tet OFF condition) Stat3F is expressed and the cells differentiate despite the presence of LIF. We thus performed microarray analysis of the transcriptome of ES cells after 16h, 24h and 48h after Tetracycline removal (OFF16; OFF24 and OFF48) respectively)and compared them to Gs2 ES cells maintained in the continuous presence of LIF and tetracyclin (LIF/ON).
Experiment type
unknown experiment type 
Contact
Citation
Microarray analysis of LIF/Stat3 transcriptional targets in embryonic stem cells. Sekkaï D, Gruel G, Herry M, Moucadel V, Constantinescu SN, Albagli O, Tronik-Le Roux D, Vainchenker W, Bennaceur-Griscelli A.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-1151.idf.txt
Sample and data relationshipE-GEOD-1151.sdrf.txt
Processed data (1)E-GEOD-1151.processed.1.zip
Array designsA-GEOD-1063.adf.txt, A-GEOD-1091.adf.txt
Links