E-GEOD-10718 - Transcription profiling by array of normal human bronchial epithelial cells after exposure to smoke

Status
Released on 6 April 2008, last updated on 14 August 2014
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (7)
Description
Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke (CS) from a typical "full flavor" American brand of cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomarkers that discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CS for 15 minutes and alterations to the transcriptome assessed at 1,2,4 and 24 hours post-CS-exposure using high-density oligonucleotide microarrays. Experiment Overall Design: Cells were exposed to smoke or air (“mock-exposed”) for 15 min, after which they were refed with fresh media and allowed to incubate for 1h, 2h, 4h or 24h. Smoke and mock samples were compared at each time point.
Experiment types
transcription profiling by array, in vitro
Contact
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-10718.idf.txt
Sample and data relationshipE-GEOD-10718.sdrf.txt
Raw data (1)E-GEOD-10718.raw.1.zip
Processed data (1)E-GEOD-10718.processed.1.zip
Array designA-AFFY-44.adf.txt
Links