E-GEOD-10580 - Transcription profiling by array of human osteosarcoma U2OS cell line conditionally expressing HA-tagged PRDM5

Status
Released on 31 January 2009, last updated on 19 September 2012
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (5)
Description
PRDM5 is a recently identified member of the PRDM family of proteins, which functions as a transcriptional repressor by recruiting histone methyltransferase G9A to DNA, and behaves as a putative tumor suppressor in different types of cancer. We investigated PRDM5 function by identifying its target genes in U2OS cells at different time points after expression of PRDM5 protein. Experiment Overall Design: We analyzed gene expression profiles of a U2OS cell line conditionally expressing HA-tagged PRDM5 (U2OS-PRDM5), where PRDM5 is under the transcriptional control of a doxycycline-inducible promoter. Expression of PRDM5 protein is detectable after 8 hours of induction with 2ug/ml doxycycline, and increases steadily up to 48 hours, after which cells begin to undergo apoptosis. U2OS cells containing the empty cloning vector (U2OS-pSG213) were used as controls for each condition.
Experiment type
transcription profiling by array 
Contact
Citation
The tumor suppressor PRDM5 regulates Wnt signaling at early stages of zebrafish development. Natalia Meani, Federica Pezzimenti, Gianluca Deflorian, Marina Mione, Myriam Alcalay.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-10580.idf.txt
Sample and data relationshipE-GEOD-10580.sdrf.txt
Raw data (1)E-GEOD-10580.raw.1.zip
Processed data (1)E-GEOD-10580.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-10580.eSet.r
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