E-GEOD-10005 - Circadian regulation of a limited set of microRNAs in Drosophila

Status
Submitted on 21 December 2007, released on 27 May 2010, last updated on 1 May 2014
Organism
Drosophila melanogaster
Samples (96)
Array (1)
Protocols (8)
Description
We used a microarray platform to survey the daily levels of 78 D. melanogaster miRNAs in adult heads of wildtype control flies and the arrhythmic clock mutant cyc01. At least 2 miRNAs were identified that showed robust daily abundance changes in control flies but not the cyc01 mutant. Keywords: microRNA, Circadian regulation yw and yw;cyc01 flies were maintained in standard media. For collections, approximately 50-100 young (2-5 day old) flies were placed in vials that were incubated at 25oC for four days in standard 12hr light-12hr dark cycles [LD; where zeitgeber time 0 (ZT0) is defined as lights-on]. Flies were collected by freezing in dry ice during the fourth LD cycle at the following times; ZT1, 7, 13 and 19. Subsequently low molecular RNAs were extracted for microarray analysis using a mirMAX microarray.
Experiment type
transcription profiling by array 
Contacts
maocheng yang <maocheng.yang@fda.hhs.gov>, Isaac Edery, JungEun Lee, Maocheng Yang, Richard W Padgett
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-10005.idf.txt
Sample and data relationshipE-GEOD-10005.sdrf.txt
Raw data (1)E-GEOD-10005.raw.1.zip
Processed data (1)E-GEOD-10005.processed.1.zip
Experiment designE-GEOD-10005.biosamples.png, E-GEOD-10005.biosamples.svg
Array designA-GEOD-6298.adf.txt
R ExpressionSetE-GEOD-10005.eSet.r
Links