E-FPMI-9 - Transcription profiling of peripheral blood monocytes from healthy humans and from those with Chronic Granulamatous Disease in response to stimulation with peptidoglycan and lipopolysaccharide

Status
Released on 5 November 2008, last updated on 1 May 2014
Organism
Homo sapiens
Samples (34)
Array (1)
Protocols (18)
Description
Persons with Chronic Granulomatous Disease, are susceptible to a narrow range of infections resulting from the failure of cells to generate toxic reactive oxygen species (ROS) required for phagocytice oxidative killing of microorganisms. For unknown reasons, many inflammatory conditions (inflammatory bowel disease, periodontal inflammation, granulomatous obstruction of the urinary and gastrointestinal tract and “sterile” inflammation of the lungs and other organs) are also associated with the disease. The goal of this study is to investigate the role of ROS in the regulation of inflammatory and immunity genes induced by Toll-like Receptors (TLRs). Transcriptional responses to peptidoglycan or lipopolysaccharide (TLR2/4 agonists) were evaluated at 4 and 24 hrs in peripheral blood monocytes (PBM) from three males with CGD compared to PBM from 5 healthy individuals.
Experiment types
transcription profiling by array, compound treatment, disease state, time series
Contact
Citation
ROS-deficient monocytes have aberrant gene expression that correlates with inflammatory disorders of chronic granulomatous disease. Brown KL, Bylund J, MacDonald KL, Song-Zhao GX, Elliott MR, Falsafi R, Hancock RE, Speert DP. Clin Immunol 129(1):90-102 (2008)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-FPMI-9.idf.txt
Sample and data relationshipE-FPMI-9.sdrf.txt
Raw data (1)E-FPMI-9.raw.1.zip
Experiment designE-FPMI-9.biosamples.png, E-FPMI-9.biosamples.svg
Array designA-FPMI-4.adf.txt
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