E-FPMI-6 - Transcription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours

Status
Released on 7 December 2006, last updated on 1 May 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (10)
Description
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence or absence of host defense peptide LL-37 at low physiologically relevant concentrations. Human monocytic cells THP-1 were stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours. The trends of LPS-induced altered gene expression in presence of the peptide were further validated by quantitative real-time PCR.
Experiment types
transcription profiling by array, compound treatment
Contact
Citation
Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide. Mookherjee, Neeloffer; Wilson, Heather L.; Doria, Silvana; Popowych, Yurij; Falsafi, Reza; Yu, Jie (Jessie); Li, YueXin; Veatch, Sarah; Roche, Fiona M.; Brown, Kelly L.; Brinkman, Fiona S. L.; Hokamp, Karsten; Potter, Andy; Babiuk, Lorne A.; Griebel, Philip J.; Hancock, Robert E. W. J Leukoc Biol 80(6):1563 (2006), Europe PMC 16943385
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-FPMI-6.idf.txt
Sample and data relationshipE-FPMI-6.sdrf.txt
Raw data (1)E-FPMI-6.raw.1.zip
Experiment designE-FPMI-6.biosamples.png, E-FPMI-6.biosamples.svg
Array designA-FPMI-4.adf.txt
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