E-FPMI-14 - Transcription profiling of Pseudomonas aeruginosa parental strain PAO1 and PAOQ_psrA::Tn5 knock out mutant

Status
Submitted on 3 July 2008, released on 4 July 2008, last updated on 2 June 2014
Organism
Pseudomonas aeruginosa
Samples (10)
Array (1)
Protocols (8)
Description
5 Biological repeats of bacterial strain PAO1(parent, control) were compared to 5 biological repeats of PAO1_psrA::Tn5 knockout mutant, both strains were grown in BM2-glucose media containing 2mM MgSO4. Cells were grown to mid-log phase, OD600 0.50 Cells were immediately harvested and RNA was extracted
Experiment types
transcription profiling by array, genetic modification
Contacts
Manjeet Bains <manjeet@cmdr.ubc.ca>, Irith Wiegand, Joseph B McPhee, Robert E Hancock, William J Gooderham
Citation
Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation and swarming motility of psrA in Pseudomonas aeruginosa. William Gooderham; Manjeet Bains; Joseph McPhee; Irith Wiegand; Robert Hancock. J Bacteriol  (2008)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-FPMI-14.idf.txt
Sample and data relationshipE-FPMI-14.sdrf.txt
Raw data (1)E-FPMI-14.raw.1.zip
Experiment designE-FPMI-14.biosamples.png, E-FPMI-14.biosamples.svg
Array designA-JCVI-28.adf.txt
Links