E-FLYC-4 - Transcription profiling of Drosophila cell lines S3, Kc and S3
Released on 7 May 2004, last updated on 1 May 2014
Background: We would like to use Drosophila cell lines to investigate the transcriptional responses to Nitric Oxide donors and inhibitors. As a pilot to this we would like to compare the transcriptional profile of 3 cell lines we are using in our lab (S3, Kc and S3). This study is being done in parallel to an in vivo genetic screen to indentify required genes. This work is funded by the BBSRC. Plan: Pairwise comparisons of each cell line to the other two. Cells will be grown at low density in identical conditions. Cells will be harvested and RNA prepared in Trizol as recommended.
transcription profiling by array, cell type comparison
Ian Roberts, unknown unknown, Debashis Rana <email@example.com>