E-FLYC-4 - Transcription profiling of Drosophila cell lines S3, Kc and S3

Status
Released on 7 May 2004, last updated on 1 May 2014
Organism
Drosophila melanogaster
Samples (9)
Array (1)
Protocols (7)
Description
Background: We would like to use Drosophila cell lines to investigate the transcriptional responses to Nitric Oxide donors and inhibitors. As a pilot to this we would like to compare the transcriptional profile of 3 cell lines we are using in our lab (S3, Kc and S3). This study is being done in parallel to an in vivo genetic screen to indentify required genes. This work is funded by the BBSRC. Plan: Pairwise comparisons of each cell line to the other two. Cells will be grown at low density in identical conditions. Cells will be harvested and RNA prepared in Trizol as recommended.
Experiment types
transcription profiling by array, cell type comparison
Contacts
Ian Roberts, unknown unknown, Debashis Rana <rana@flymine.org>
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-FLYC-4.idf.txt
Sample and data relationshipE-FLYC-4.sdrf.txt
Raw data (1)E-FLYC-4.raw.1.zip
Processed data (1)E-FLYC-4.processed.1.zip
Experiment designE-FLYC-4.biosamples.png, E-FLYC-4.biosamples.svg
Array designA-FLYC-2.adf.txt
Links