E-ERAD-84 - RNA-seq of zebrafish embryos with morphologically mutant phenotypes associated with knockout alleles, Zebrafish Mutation Project

Status
Released on 13 May 2013, last updated on 1 October 2013
Organism
Danio rerio
Samples (34)
Protocols (3)
Description
Total RNA was extracted from wild type and mutant zebrafish embryos. Double stranded cDNA representing the 3' ends of transcripts was made by a variety of methods, including polyT priming and 3' pull down on magentic beads. Some samples included indexing test experiments where a sequence barcode was placed within one of the sequence reads. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_phD This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Experiment types
RNA-seq of coding RNA, development or differentiation design, genotype design
Contact
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-ERAD-84.idf.txt
Sample and data relationshipE-ERAD-84.sdrf.txt
Links