E-ERAD-242 - RNA-Seq of human THP1 cells in response to Staphylococcus aureus exposure

Status
Released on 12 May 2014, last updated on 15 May 2014
Organism
Homo sapiens
Samples (9)
Protocols (2)
Description
Biological replicates of THP1 cells incubated with live wild type S. aureus, THP1 cells incubated with dead wild type S. aureus and THP1 cells incubated with saeS- S. aureus were collected and their RNA were isolated via the Qiagen RNA Isolation Kit. The RNA were treated with RNAse free DNase and eluted in RNase-free water. The RNA concentration was assessed using a NanoDrop spectrophotometer, while RNA integrity (RIN >8) were obtained for all samples by Agilent 2100 Bioanalyzer. We aim to sequence 5GBp per replicate and all samples on a single lane on an Illumina platform.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Experiment types
RNA-seq of coding RNA, stimulus or stress design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-ERAD-242.idf.txt
Sample and data relationshipE-ERAD-242.sdrf.txt
Links